9
Sep
PCR Machine Features Explained: Gradient, Multiplexing, and Throughput for Australian Labs
The performance of a PCR machine depends not only on its build quality but also on the features that support accuracy and workflow efficiency. For Australian laboratories in education, diagnostics, and research, understanding key thermal cycler features like gradient capability, multiplexing, and throughput helps ensure the right investment. At LabChoice Australia, we guide labs through these choices, offering PCR machines that meet international standards while providing local service and support.
Gradient Function: Flexibility in Annealing Temperatures
PCR relies on precise temperature cycling, and annealing temperature can make or break an assay. A gradient thermal cycler allows multiple annealing temperatures to be tested across a single run, saving time and reagents.
- Ideal for assay optimisation in teaching and research labs.
- Reduces trial-and-error across multiple instruments.
- Ensures reproducibility when scaling experiments.
Australian genetics and molecular biology labs benefit greatly from this function, especially when developing custom assays.
Multiplexing Capability: More Data in Less Time
Multiplex PCR enables detection of multiple DNA targets in a single reaction. This is essential for high-value diagnostics and complex workflows.
- Clinical labs: Detect multiple pathogens simultaneously.
- Food and environmental labs: Screen for contaminants in one test.
- Research labs: Analyse several genes in parallel.
Modern qPCR thermal cyclers with advanced optics and software make multiplexing both accurate and cost-efficient.
Throughput: Matching Machine Capacity to Lab Needs
Throughput refers to the number of samples a PCR machine can process in one run.
| Lab Type | Recommended Capacity | Example Application |
|---|---|---|
| Teaching / small research labs | 48–96 wells | Student experiments, pilot projects |
| Medium throughput labs | 96–192 wells | Routine diagnostics, research runs |
| High-throughput facilities | 384 wells+ | Large-scale diagnostics, QC labs |
LabChoice offers a range of options to suit schools, universities, and high-throughput clinical labs, ensuring every facility can scale efficiently.
Comparison: Standard vs Advanced PCR Features
| Feature | Standard PCR Machine | Advanced LabChoice PCR Machine |
|---|---|---|
| Gradient function | Not available | Full gradient across block |
| Multiplexing | Single-target only | Multi-target detection (qPCR ready) |
| Throughput | 48–96 wells | Up to 384 wells |
| Software | Basic interface | Real-time monitoring & data export |
| Compliance | General performance | ISO 13485 & ASTM E2108 certified |
This comparison highlights how advanced LabChoice PCR systems deliver higher flexibility, accuracy, and compliance than basic models often sold in Australia.
FAQs
Q1: What is the benefit of a gradient PCR machine?
It allows labs to test different annealing temperatures in one run, saving time and reagents.
Q2: Do all PCR machines support multiplexing?
No. Multiplexing requires advanced qPCR systems with specialised optics and software.
Q3: How do I choose the right throughput for my lab?
Match sample demand with machine capacity — schools often use 96-well models, while diagnostic labs may need 384-well systems.
Q4: Do LabChoice PCR machines meet compliance standards?
Yes. All LabChoice systems comply with ISO 13485 and ASTM E2108, ensuring global performance benchmarks.
Refined References
- ISO 13485: Medical Devices – Quality Management Systems. ISO
- ASTM E2108: PCR Optical Performance Standards. ASTM International
- Australian Government – Molecular Diagnostics Guidelines. Health.gov.au
- National Center for Biotechnology Information – PCR Methodologies. NCBI
